An organic-inorganic hybrid scaffold with honeycomb-like structures enabled by one-step self-assembly-driven electrospinning

Electrospun organic/inorganic hybrid scaffolds have been appealing in tissue regeneration owing to the integrated physicochemical and biological performances. However, conventional electrospun with non-woven structures usually failed enable deep cell infiltration due densely stacked layers among fibers. Herein, through self-assembly-driven electrospinning, a polyhydroxybutyrate/poly(ε-caprolactone)/58S sol-gel bioactive glass (PHB/PCL/58S) scaffold honeycomb-like was prepared by manipulating solution composition concentration during one-step electrospinning process. The mechanisms enabling formation of self-assembled were investigated comparative studies using Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) thermogravimetric analysis (TGA) between PHB/PCL/58S PHB/PCL/sol-gel silica systems. obtained structure built up from nanofibers an average diameter 370 nm showed bimodal distribution pores: large polygonal pores hundreds micrometers within honeycomb-cells irregular ranging around few micrometers. cell-materials interactions further studied culturing MG-63 osteoblast-like cells for 7 days. Cell viability, morphology comparatively as well. While merely proliferated on surface structures, extensive proliferation 100–200 μm into structure. Moreover, cellular spatial organization readily regulated pattern Overall, newly may integrate enhanced osteogenicity originating components, improved cell-material brought structure, making new promising candidate regeneration.